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anti ttn titin monoclonal antibody  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank anti ttn titin monoclonal antibody
    (A) Outline of cell isolation and FACS of SSC-A Hi /GFP Hi ventricular cardiomyocytes from adult male zebrafish. (B) Representative laser confocal z-stack images of a sorted cardiomyocyte showing the colocalization of two sarcomeric proteins. α-Actinin staining is shown in cyan hot, <t>Titin</t> in orange hot, and nuclear staining in grey. The image on the right shows a magnified (3x zoom) area. (C) Representative laser confocal z-stack images of a sorted cardiomyocyte showing the sarcomeric localization of α-Actinin and myomesin. α-Actinin staining is shown in cyan hot, Myomesin in orange hot, and nuclear staining in grey. The images on the right (c’ and c’’) show 3x magnified regions highlighting the M-bands and Z-discs. (D) Normalized fluorescence intensity profile showing sarcomeric α-Actinin and Myomesin staining along the depicted line shown in c’’. The M-bands are labeled by Myomesin and the Z-discs by α-Actinin.
    Anti Ttn Titin Monoclonal Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 146 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ttn titin monoclonal antibody/product/Developmental Studies Hybridoma Bank
    Average 95 stars, based on 146 article reviews
    anti ttn titin monoclonal antibody - by Bioz Stars, 2026-03
    95/100 stars

    Images

    1) Product Images from "Proteo-transcriptomics and morphometrics of teleost cardiac cells define regulatory networks and exercise-induced cardiomyocyte hypertrophy and hyperplasia"

    Article Title: Proteo-transcriptomics and morphometrics of teleost cardiac cells define regulatory networks and exercise-induced cardiomyocyte hypertrophy and hyperplasia

    Journal: bioRxiv

    doi: 10.64898/2026.01.11.698907

    (A) Outline of cell isolation and FACS of SSC-A Hi /GFP Hi ventricular cardiomyocytes from adult male zebrafish. (B) Representative laser confocal z-stack images of a sorted cardiomyocyte showing the colocalization of two sarcomeric proteins. α-Actinin staining is shown in cyan hot, Titin in orange hot, and nuclear staining in grey. The image on the right shows a magnified (3x zoom) area. (C) Representative laser confocal z-stack images of a sorted cardiomyocyte showing the sarcomeric localization of α-Actinin and myomesin. α-Actinin staining is shown in cyan hot, Myomesin in orange hot, and nuclear staining in grey. The images on the right (c’ and c’’) show 3x magnified regions highlighting the M-bands and Z-discs. (D) Normalized fluorescence intensity profile showing sarcomeric α-Actinin and Myomesin staining along the depicted line shown in c’’. The M-bands are labeled by Myomesin and the Z-discs by α-Actinin.
    Figure Legend Snippet: (A) Outline of cell isolation and FACS of SSC-A Hi /GFP Hi ventricular cardiomyocytes from adult male zebrafish. (B) Representative laser confocal z-stack images of a sorted cardiomyocyte showing the colocalization of two sarcomeric proteins. α-Actinin staining is shown in cyan hot, Titin in orange hot, and nuclear staining in grey. The image on the right shows a magnified (3x zoom) area. (C) Representative laser confocal z-stack images of a sorted cardiomyocyte showing the sarcomeric localization of α-Actinin and myomesin. α-Actinin staining is shown in cyan hot, Myomesin in orange hot, and nuclear staining in grey. The images on the right (c’ and c’’) show 3x magnified regions highlighting the M-bands and Z-discs. (D) Normalized fluorescence intensity profile showing sarcomeric α-Actinin and Myomesin staining along the depicted line shown in c’’. The M-bands are labeled by Myomesin and the Z-discs by α-Actinin.

    Techniques Used: Cell Isolation, Staining, Fluorescence, Labeling

    (A) Outline of adult male zebrafish ventricle isolation, dissociation, cytospin, and subsequent laser confocal imaging. (B) Representative laser confocal images of GFP+ cardiomyocytes showing intact and high-quality cells with visible sarco-meres. GFP fluorescence is shown in cyan, while nuclei are shown in yellow. (C) Representative laser confocal images of GFP+ cardiomyocytes showing intact cells with variable morphological shapes and sizes. Four main morphological features are highlighted, including rounded, rod-shaped, multipolar, and elongated cardiomyocytes. (D) Outline of cell isolation and FACS of GFP+ ventricular cardiomyocytes from adult male zebrafish. (E) Representative laser confocal images of FACS-isolated cardiomyocytes showing α-Actinin and myomesin staining. α-Actinin staining is shown in cyan hot, myomesin in orange hot, and nuclear staining in grey. The image on the right shows a resulting mask from the staining image on the left using Fiji. (F) Masks of randomly selected cardiomyocytes from (E) showing marked differences in the morphology and shape of cardiomyocytes. (G) Quantitative comparisons of perimeter, MinFeret, Circularity, and Aspect Ratio descriptors are shown (each group representing N = 6), revealing heterogeneity in male cardiomyocyte size and shape. (H) Outline of adult male zebrafish ventricle isolation, dissociation, and live-cell acoustic flow cytometry and bright field imaging of the GFP Hi SSC-A Hi cardiomyocyte cluster. Cells were run without fixation. (I) Representative bright field images of GFP Hi SSC-A Hi cardiomyocytes obtained using Attune CytPix acoustic focus.
    Figure Legend Snippet: (A) Outline of adult male zebrafish ventricle isolation, dissociation, cytospin, and subsequent laser confocal imaging. (B) Representative laser confocal images of GFP+ cardiomyocytes showing intact and high-quality cells with visible sarco-meres. GFP fluorescence is shown in cyan, while nuclei are shown in yellow. (C) Representative laser confocal images of GFP+ cardiomyocytes showing intact cells with variable morphological shapes and sizes. Four main morphological features are highlighted, including rounded, rod-shaped, multipolar, and elongated cardiomyocytes. (D) Outline of cell isolation and FACS of GFP+ ventricular cardiomyocytes from adult male zebrafish. (E) Representative laser confocal images of FACS-isolated cardiomyocytes showing α-Actinin and myomesin staining. α-Actinin staining is shown in cyan hot, myomesin in orange hot, and nuclear staining in grey. The image on the right shows a resulting mask from the staining image on the left using Fiji. (F) Masks of randomly selected cardiomyocytes from (E) showing marked differences in the morphology and shape of cardiomyocytes. (G) Quantitative comparisons of perimeter, MinFeret, Circularity, and Aspect Ratio descriptors are shown (each group representing N = 6), revealing heterogeneity in male cardiomyocyte size and shape. (H) Outline of adult male zebrafish ventricle isolation, dissociation, and live-cell acoustic flow cytometry and bright field imaging of the GFP Hi SSC-A Hi cardiomyocyte cluster. Cells were run without fixation. (I) Representative bright field images of GFP Hi SSC-A Hi cardiomyocytes obtained using Attune CytPix acoustic focus.

    Techniques Used: Isolation, Imaging, Fluorescence, Cell Isolation, Staining, Flow Cytometry



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    (A) Outline of cell isolation and FACS of SSC-A Hi /GFP Hi ventricular cardiomyocytes from adult male zebrafish. (B) Representative laser confocal z-stack images of a sorted cardiomyocyte showing the colocalization of two sarcomeric proteins. α-Actinin staining is shown in cyan hot, <t>Titin</t> in orange hot, and nuclear staining in grey. The image on the right shows a magnified (3x zoom) area. (C) Representative laser confocal z-stack images of a sorted cardiomyocyte showing the sarcomeric localization of α-Actinin and myomesin. α-Actinin staining is shown in cyan hot, Myomesin in orange hot, and nuclear staining in grey. The images on the right (c’ and c’’) show 3x magnified regions highlighting the M-bands and Z-discs. (D) Normalized fluorescence intensity profile showing sarcomeric α-Actinin and Myomesin staining along the depicted line shown in c’’. The M-bands are labeled by Myomesin and the Z-discs by α-Actinin.
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    Image Search Results


    (A) Outline of cell isolation and FACS of SSC-A Hi /GFP Hi ventricular cardiomyocytes from adult male zebrafish. (B) Representative laser confocal z-stack images of a sorted cardiomyocyte showing the colocalization of two sarcomeric proteins. α-Actinin staining is shown in cyan hot, Titin in orange hot, and nuclear staining in grey. The image on the right shows a magnified (3x zoom) area. (C) Representative laser confocal z-stack images of a sorted cardiomyocyte showing the sarcomeric localization of α-Actinin and myomesin. α-Actinin staining is shown in cyan hot, Myomesin in orange hot, and nuclear staining in grey. The images on the right (c’ and c’’) show 3x magnified regions highlighting the M-bands and Z-discs. (D) Normalized fluorescence intensity profile showing sarcomeric α-Actinin and Myomesin staining along the depicted line shown in c’’. The M-bands are labeled by Myomesin and the Z-discs by α-Actinin.

    Journal: bioRxiv

    Article Title: Proteo-transcriptomics and morphometrics of teleost cardiac cells define regulatory networks and exercise-induced cardiomyocyte hypertrophy and hyperplasia

    doi: 10.64898/2026.01.11.698907

    Figure Lengend Snippet: (A) Outline of cell isolation and FACS of SSC-A Hi /GFP Hi ventricular cardiomyocytes from adult male zebrafish. (B) Representative laser confocal z-stack images of a sorted cardiomyocyte showing the colocalization of two sarcomeric proteins. α-Actinin staining is shown in cyan hot, Titin in orange hot, and nuclear staining in grey. The image on the right shows a magnified (3x zoom) area. (C) Representative laser confocal z-stack images of a sorted cardiomyocyte showing the sarcomeric localization of α-Actinin and myomesin. α-Actinin staining is shown in cyan hot, Myomesin in orange hot, and nuclear staining in grey. The images on the right (c’ and c’’) show 3x magnified regions highlighting the M-bands and Z-discs. (D) Normalized fluorescence intensity profile showing sarcomeric α-Actinin and Myomesin staining along the depicted line shown in c’’. The M-bands are labeled by Myomesin and the Z-discs by α-Actinin.

    Article Snippet: Conjugated and primary antibodies used in this study include anti-Cardiac Troponin T (cTnT) Mouse Monoclonal Antibody (13-11, 1:500; ThermoFisher Scientific); Alexa Fluor® 647 Mouse anti-Cardiac Troponin T (cTnT), (1:250; BD Biosciences), PE Mouse anti-Cardiac Troponin T (cTnT), (1:250; BD Biosciences), anti-α-Actinin (Sarcomeric) Vio® R667, REAfinityTM (1:200; Miltenyi Biotec), anti-TTN (Titin) monoclonal antibody (M07), clone 2F12 (mouse, 1:250, Abnova), anti-Myomesin (mouse, mMaC myomesin B4, 1:200, DSHB).

    Techniques: Cell Isolation, Staining, Fluorescence, Labeling

    (A) Outline of adult male zebrafish ventricle isolation, dissociation, cytospin, and subsequent laser confocal imaging. (B) Representative laser confocal images of GFP+ cardiomyocytes showing intact and high-quality cells with visible sarco-meres. GFP fluorescence is shown in cyan, while nuclei are shown in yellow. (C) Representative laser confocal images of GFP+ cardiomyocytes showing intact cells with variable morphological shapes and sizes. Four main morphological features are highlighted, including rounded, rod-shaped, multipolar, and elongated cardiomyocytes. (D) Outline of cell isolation and FACS of GFP+ ventricular cardiomyocytes from adult male zebrafish. (E) Representative laser confocal images of FACS-isolated cardiomyocytes showing α-Actinin and myomesin staining. α-Actinin staining is shown in cyan hot, myomesin in orange hot, and nuclear staining in grey. The image on the right shows a resulting mask from the staining image on the left using Fiji. (F) Masks of randomly selected cardiomyocytes from (E) showing marked differences in the morphology and shape of cardiomyocytes. (G) Quantitative comparisons of perimeter, MinFeret, Circularity, and Aspect Ratio descriptors are shown (each group representing N = 6), revealing heterogeneity in male cardiomyocyte size and shape. (H) Outline of adult male zebrafish ventricle isolation, dissociation, and live-cell acoustic flow cytometry and bright field imaging of the GFP Hi SSC-A Hi cardiomyocyte cluster. Cells were run without fixation. (I) Representative bright field images of GFP Hi SSC-A Hi cardiomyocytes obtained using Attune CytPix acoustic focus.

    Journal: bioRxiv

    Article Title: Proteo-transcriptomics and morphometrics of teleost cardiac cells define regulatory networks and exercise-induced cardiomyocyte hypertrophy and hyperplasia

    doi: 10.64898/2026.01.11.698907

    Figure Lengend Snippet: (A) Outline of adult male zebrafish ventricle isolation, dissociation, cytospin, and subsequent laser confocal imaging. (B) Representative laser confocal images of GFP+ cardiomyocytes showing intact and high-quality cells with visible sarco-meres. GFP fluorescence is shown in cyan, while nuclei are shown in yellow. (C) Representative laser confocal images of GFP+ cardiomyocytes showing intact cells with variable morphological shapes and sizes. Four main morphological features are highlighted, including rounded, rod-shaped, multipolar, and elongated cardiomyocytes. (D) Outline of cell isolation and FACS of GFP+ ventricular cardiomyocytes from adult male zebrafish. (E) Representative laser confocal images of FACS-isolated cardiomyocytes showing α-Actinin and myomesin staining. α-Actinin staining is shown in cyan hot, myomesin in orange hot, and nuclear staining in grey. The image on the right shows a resulting mask from the staining image on the left using Fiji. (F) Masks of randomly selected cardiomyocytes from (E) showing marked differences in the morphology and shape of cardiomyocytes. (G) Quantitative comparisons of perimeter, MinFeret, Circularity, and Aspect Ratio descriptors are shown (each group representing N = 6), revealing heterogeneity in male cardiomyocyte size and shape. (H) Outline of adult male zebrafish ventricle isolation, dissociation, and live-cell acoustic flow cytometry and bright field imaging of the GFP Hi SSC-A Hi cardiomyocyte cluster. Cells were run without fixation. (I) Representative bright field images of GFP Hi SSC-A Hi cardiomyocytes obtained using Attune CytPix acoustic focus.

    Article Snippet: Conjugated and primary antibodies used in this study include anti-Cardiac Troponin T (cTnT) Mouse Monoclonal Antibody (13-11, 1:500; ThermoFisher Scientific); Alexa Fluor® 647 Mouse anti-Cardiac Troponin T (cTnT), (1:250; BD Biosciences), PE Mouse anti-Cardiac Troponin T (cTnT), (1:250; BD Biosciences), anti-α-Actinin (Sarcomeric) Vio® R667, REAfinityTM (1:200; Miltenyi Biotec), anti-TTN (Titin) monoclonal antibody (M07), clone 2F12 (mouse, 1:250, Abnova), anti-Myomesin (mouse, mMaC myomesin B4, 1:200, DSHB).

    Techniques: Isolation, Imaging, Fluorescence, Cell Isolation, Staining, Flow Cytometry